Masu binciken Dutch sun haɗu da CRISPR da bioluminescence a cikin gwajin gwaji doncututtuka masu yaduwa

Wani sabon furotin da aka haɓaka na dare zai iya hanzarta da sauƙaƙe gano cututtukan ƙwayoyin cuta, a cewar masu bincike a Netherlands.
Binciken nasu, wanda aka buga Laraba a cikin ACS Publications, ya bayyana hanya mai mahimmanci, mataki ɗaya don yin saurin nazarin kwayoyin nucleic acid da bayyanar su ta amfani da sunadaran sunadaran shuɗi ko kore.
Gano ƙwayoyin cuta ta hanyar gano alamun yatsun acid ɗin su shine mabuɗin dabarun bincike na asibiti, binciken ilimin halittu, da kula da lafiyar abinci da muhalli.Gwaje-gwajen da aka yi amfani da su da yawa na jimlar polymerase sarkar amsa (PCR) suna da matukar kulawa, amma suna buƙatar shirye-shiryen samfurin nagartaccen ko fassarar sakamako, yana sa su zama marasa amfani ga wasu saitunan kiwon lafiya ko iyakantattun saitunan albarkatu.
Wannan rukunin daga Netherlands shine sakamakon haɗin gwiwa tsakanin masana kimiyya daga jami'o'i da asibitoci don haɓaka hanyar bincike mai sauri, šaukuwa da sauƙi don amfani da kwayoyin acid nucleic acid wanda za'a iya amfani da shi a wurare daban-daban.
An yi musu wahayi ta hanyar walƙiya na wuta, hasken wuta, da ƙananan taurari na phytoplankton na ruwa, duk wani abu mai ƙarfi da ake kira bioluminescence.Wannan sakamako mai haske-a cikin duhu yana haifar da halayen sinadarai wanda ya ƙunshi furotin luciferase.Masanan kimiyya sun haɗa sunadaran luciferase cikin na'urori masu auna firikwensin da ke fitar da haske don sauƙaƙe dubawa lokacin da suka sami manufa.Duk da yake wannan ya sa waɗannan na'urori masu auna firikwensin su dace don gano ma'anar kulawa, a halin yanzu ba su da babban hazaka da ake buƙata don gwaje-gwajen bincike na asibiti.Yayin da hanyar gyare-gyare na CRISPR na iya samar da wannan damar, yana buƙatar matakai da yawa da ƙarin kayan aiki na musamman don gano siginar rauni wanda zai iya kasancewa a cikin hadaddun, samfurori masu hayaniya.
Masu bincike sun sami hanyar haɗa furotin mai alaƙa da CRISPR tare da siginar bioluminescent wanda za'a iya gano shi tare da kyamarar dijital mai sauƙi.Don tabbatar da cewa akwai isassun samfurin RNA ko DNA don bincike, masu binciken sun yi recombinase polymerase amplification (RPA), wata hanya mai sauƙi wacce ke aiki a koyaushe a yanayin zafi na kusan 100 ° F.Sun ɓullo da wani sabon dandali mai suna Luminescent Nucleic Acid Sensor (LUNAS), wanda a cikinsa sunadaran CRISPR/Cas9 guda biyu sun keɓance ga sassa daban-daban na ƙwayoyin cuta na kwayar cuta, kowanne tare da guntun luciferase na musamman da aka haɗe zuwa sama.
Lokacin da takamaiman kwayar cutar kwayar cutar kwayar cutar kwayar cutar da masu binciken ke bincikar su ta kasance, sunadaran CRISPR/Cas9 guda biyu suna ɗaure zuwa jerin acid nucleic manufa;sun kasance kusa da kusanci, suna barin furotin luciferase mara kyau ya samar kuma ya fitar da haske shuɗi a gaban wani sinadari na sinadarai..Don yin lissafin abin da ake cinyewa a cikin wannan tsari, masu binciken sun yi amfani da yanayin sarrafawa wanda ke fitar da hasken kore.Bututu mai canza launi daga kore zuwa shuɗi yana nuna sakamako mai kyau.
Masu binciken sun gwada dandalin su ta hanyar haɓaka gwajin RPA-LUNAS, wanda ke ganowaSARS-CoV-2 RNAba tare da keɓewar RNA mai wahala ba, kuma ya nuna aikin binciken sa akan samfuran swab na nasopharyngeal dagaCUTAR COVID 19marasa lafiya.RPA-LUNAS ya sami nasarar gano SARS-CoV-2 a cikin mintuna 20 a cikin samfurori tare da nauyin kwayar cutar RNA mai ƙarancin kwafi 200/μL.
Masu binciken sun yi imanin cewa gwajin nasu zai iya gano wasu ƙwayoyin cuta cikin sauƙi da inganci."RPA-LUNAS yana da kyau don gwajin cututtukan cututtukan da ke da alaƙa," sun rubuta.