Amfani da Niyya

Gwajin Dengue NS1 Ag-IgG/IgM Combo shine saurin chromatographic immunoassay don gano ingancin ƙwayoyin rigakafi (IgG da IgM) da ƙwayar cuta ta dengue NS1 antigen zuwa cutar dengue a cikin Serum ko Plasma don taimakawa wajen gano cutar kamuwa da cuta ta Dengue.

[TAKATAI]

Zazzabin Dengue cuta ce mai saurin kamuwa da cuta mai saurin kamuwa da kwayar cutar dengue da sauro ke yadawa.Kwayar cutar Dengue na iya haifar da kamuwa da cuta, zazzabin dengue, zazzabin jini na dengue, zazzabin jini na dengue.Yawan bayyanar cututtuka na zazzabin dengue sun haɗa da farawa kwatsam, zazzabi mai zafi, ciwon kai, tsoka mai tsanani, kashi da ciwon haɗin gwiwa, kurjin fata, yanayin zubar jini, haɓakar kumburin lymph, rage yawan adadin jinin jini, thrombocytopenia da sauransu a wasu marasa lafiya.Wannan cuta m yana a wurare masu zafi da kuma subtropical yankin shahararsa, saboda wannan cuta ana daukar kwayar cutar ta hanyar sauro Aides, dalilin shahararsa yana da wasu seasonally, zama a kowace shekara yawanci a watan Mayu ~ Nuwamba, kololuwa ne a Yuli ~ Satumba.A cikin sabon yankin da ake fama da cutar, yawancin jama'a na iya kamuwa da cutar, amma abin da ya faru ya fi girma, a yankin da cutar ta fi kamari, yawanci yara ne.

Ka'ida

Gwajin Dengue NS1 Ag-IgG/IgM Combo shine ingantacciyar membrane tsiri bisa immunoassay don gano ƙwayoyin ƙwayoyin cuta na dengue (IgG da IgM) da kwayar cutar dengue NS1 antigen a cikin Serum ko Plasma.

Don Gwajin IgG/IgM: Na'urar gwajin ta ƙunshi: 1) pad mai launi mai launin burgundy mai ɗauke da antigens recombinant envelope antigens conjugated da Colloid zinariya (dengue conjugates), 2) wani nitrocellulose membrane tsiri dauke da biyu gwajin Lines (T1 da T2 Lines) da kuma layin sarrafawa (layin C).An riga an rufe layin T1 tare da antibody don gano IgM anti-dengue, layin T2 an rufe shi da antibody don gano IgG anti-dengue.Lokacin da aka ba da isasshen adadin samfurin gwaji a cikin rijiyar samfurin kaset ɗin, samfurin yana ƙaura ta hanyar aikin capillary a cikin kaset ɗin.IgG anti-dengue, idan akwai a cikin samfurin, zai ɗaure zuwa dengue conjugates.Immunocomplex an kama shi ta hanyar reagent wanda aka riga aka lullube akan rukunin T2, yana samar da layin T2 mai launin burgundy, yana nuna sakamakon gwajin IgG mai kyau na dengue kuma yana ba da shawarar kamuwa da cuta kwanan nan ko maimaitawa.IgM anti-dengue idan akwai a cikin samfurin zai ɗaure ga dengue conjugates.An kama immunocomplex ta hanyar reagent mai rufi akan layin T1, yana samar da layin T1 mai launin burgundy, yana nuna sakamakon gwajin IgM mai inganci kuma yana ba da shawarar sabon kamuwa da cuta.Rashin kowane layin T (T1 da T2) yana nuna sakamako mara kyau.

Don Gwajin NS1: A cikin wannan hanyar gwajin, anti-Dengue NS1 antibody ba shi da motsi a cikin yankin layin gwajin na kaset.Bayan an sanya cikakken jinin /Magunguna / Plasma a cikin samfurin da kyau, yana amsawa da ƙwayoyin cuta na anti-Dengue NS1 antibody waɗanda aka shafa a kushin samfurin.Wannan cakuda yana ƙaura ta hanyar chromatographically tare da tsayin tsiri na gwaji kuma yana hulɗa tare da anti-Dengue NS1 antibody mara motsi.Idan samfurin ya ƙunshi kwayar cutar dengue NS1 antigen, wani layi mai launi zai bayyana a yankin layin gwajin yana nuna sakamako mai kyau.Idan samfurin bai ƙunshi kwayar cutar dengue NS1 antigen ba, layin launi ba zai bayyana a wannan yanki yana nuna mummunan sakamako ba.

Don yin aiki azaman sarrafa tsari, layi mai launi koyaushe zai bayyana a yankin layin sarrafawa wanda ke nuna cewa an ƙara ƙarar samfurin daidai kuma an sami wicking membrane.

Adana da Kwanciyar hankali

Ajiye kamar yadda aka kunshe a cikin jakar da aka rufe a zafin daki ko firiji (4-30 ℃ ko 40-86 ℉).Na'urar gwajin ta tsaya tsayin daka ta ranar ƙarewar da aka buga akan jakar da aka hatimi.

Dole ne gwajin ya kasance a cikin jakar da aka rufe har sai an yi amfani da shi.

Tarin Samfurin da Shirye

1. Ana iya yin gwajin Dengue NS1 Ag-IgG/IgM Combo Test akan Serum ko Plasma.

2. Don tattara duka jini, jini ko samfuran plasma bin hanyoyin gwajin asibiti na yau da kullun.

3. Ya kamata a yi gwaji nan da nan bayan an tattara samfurin.Kar a bar samfuran a zafin jiki na tsawon lokaci.Don ajiya na dogon lokaci, samfuran samfuran yakamata a kiyaye su ƙasa -20 ℃.

4. Kawo samfurori zuwa zafin jiki kafin gwaji.Daskararrun samfuran dole ne a narke gaba ɗaya kuma a gauraye su da kyau kafin gwaji.Kada a daskare samfuran kuma a narke akai-akai.

Tsarin Gwaji

Bada gwajin, samfuri, buffer da/ko sarrafawa don isa ga zafin dakin 15-30℃ (59-86℉) kafin gwaji.

1. Kawo jakar zuwa dakin zafin jiki kafin bude shi.Cire na'urar gwajin daga jakar da aka rufe kuma amfani da shi da wuri-wuri.Sanya na'urar gwajin a kan tsaftataccen wuri mai ma'auni.

2. Don Gwajin IgG/IgM: Riƙe dropper a tsaye kuma canja wurin digo 1 na samfurin (kimanin 10μl) zuwa samfurin rijiyar (S) na na'urar gwajin, sannan ƙara digo 2 na buffer (kimanin 70μl) kuma fara mai ƙidayar lokaci.Dubi hoton da ke ƙasa.

3. Don Gwajin NS1: Riƙe dropper a tsaye kuma canja wurin 8 ~ 10 digo na jini ko plasma (kimanin 100μl) zuwa samfurin da kyau (S) na na'urar gwajin, sannan fara mai ƙidayar lokaci.Dubi hoton da ke ƙasa.

4. Jira layin (s) masu launi ya bayyana.Karanta sakamako a minti 15.Kar a fassara sakamakon bayan mintuna 20.

Tafsirin Sakamako

Mai kyau:

Don Gwajin IgG/IgM: Layin sarrafawa kuma aƙalla layin gwaji ɗaya ya bayyana akan membrane.Bayyanar layin gwajin T2 yana nuna kasancewar takamaiman ƙwayoyin rigakafin IgG na dengue.Bayyanar layin gwajin T1 yana nuna kasancewar takamaiman ƙwayoyin rigakafin IgM na dengue.Kuma idan duka layin T1 da T2 sun bayyana, yana nuna cewa kasancewar duka ƙwayoyin cuta na IgG da IgM na dengue.Ƙananan ƙaddamarwar antibody shine, mafi raunin layin sakamakon shine.

Don Gwajin NS1: Layuka biyu sun bayyana.Layi ɗaya ya kamata ya bayyana koyaushe a cikin yankin layin sarrafawa (C), wani kuma wani layin da ya bayyana mai launi ya bayyana a yankin layin gwaji.

Mara kyau:

Layi mai launi ɗaya yana bayyana a cikin yankin sarrafawa(C) .Babu wani layi mai launi da ya bayyana a yankin layin gwaji.

Ba daidai ba: Layin sarrafawa ya kasa bayyana.Rashin isassun samfurin ƙira ko dabarun ƙa'ida ba daidai ba shine mafi kusantar dalilai na gazawar layin sarrafawa.Bita tsarin kuma maimaita gwajin tare da sabuwar na'urar gwaji.Idan matsalar ta ci gaba, daina amfani da kayan gwajin nan da nan kuma tuntuɓi mai rarraba na gida.