TAKAITACCEN

Babban hanyar gano kamuwa da cuta tare da HCV ita ce lura da kasancewar ƙwayoyin rigakafi ga ƙwayoyin cuta ta hanyar EIA sannan tabbatarwa tare da Western Blot.Gwajin HCV mataki ɗaya mataki ne mai sauƙi, gwajin inganci na gani wanda ke gano ƙwayoyin rigakafi a cikin Jikin ɗan adam gabaɗaya/magani/plasma.Gwajin ya dogara ne akan immunochromatography kuma yana iya ba da sakamako a cikin mintuna 15.

AMFANI DA NUFIN

Gwajin HCV Mataki ɗaya shine haɓakar Colloidal Gold, saurin Immunochromatoraphic Assay don gano ƙwararrun ƙwayoyin rigakafin cutar Hepatitis C (HCV) a cikin Dukan Jini na ɗan adam /Serum / Plasma.Wannan gwajin gwajin gwaji ne kuma dole ne a tabbatar da duk abubuwan da suka dace ta amfani da madadin gwajin kamar Western Blot.Anyi nufin gwajin don amfanin ƙwararrun Kiwon lafiya kawai.Dukkan gwaje-gwajen da sakamakon gwajin ana nufin ƙwararrun likitoci da na shari'a ne kawai su yi amfani da su, sai dai idan an ba da izini ta hanyar ƙa'ida a ƙasar amfani.Bai kamata a yi amfani da gwajin ba tare da kulawar da ta dace ba.

KA'IDAR HANYA

Gwajin yana farawa tare da samfurin da aka yi amfani da shi a kan rijiyar samfurin da kuma ƙari na samfurin da aka ba da shi nan da nan.HCV antigen-Colloidal Zinare conjugate da aka saka a cikin samfurin kushin yana amsawa tare da maganin rigakafi na HCV da ke cikin jini ko plasma, yana samar da hadadden antibody/HCV.Yayin da aka ba da izinin cakuda don yin ƙaura tare da tsiri na gwaji, haɗin haɗin haɗin gwiwa/HCV yana kama da furotin mai ɗaure antibody A wanda ba ya motsi a kan membrane da ke samar da band mai launi a yankin gwajin.Samfura mara kyau baya samar da layin gwaji saboda rashi na Colloidal Gold conjugate/HCV antibody complex.Antigens da aka yi amfani da su a cikin gwajin sunadaran sunadaran sake haɗawa da suka yi daidai da yankuna na HCV masu ƙarfi sosai.Ƙungiyar sarrafawa mai launi a cikin yankin sarrafawa yana bayyana a ƙarshen aikin gwajin ba tare da la'akari da sakamakon gwajin ba.Wannan rukunin sarrafawa shine sakamakon haɗin gwal na Colloidal Gold conjugate daure zuwa anti-HCV antibody wanda ba ya motsi akan membrane.Layin sarrafawa yana nuna cewa haɗin gwal na Colloidal Gold yana aiki.Rashin ƙungiyar sarrafawa yana nuna cewa gwajin ba daidai ba ne.

REAgents DA KAYAN DA AKA BAYAR

Gwajin na'urar ɗaiɗaiku jakar da aka yi da abin wankewa

• Fitar filastik.

• Samfurin Diluent

• Saka kunshin

KAYAN DA AKE BUKATA AMMA BA'A SAMU BA

Ingantattun sarrafawa da mara kyau (akwai a matsayin wani abu dabam)

MATSAYI & KWANTA

Dole ne a adana kayan gwajin a 2-30 ℃ a cikin jakar da aka rufe kuma a ƙarƙashin yanayin bushewa.

GARGADI DA TSIRA

1) Duk kyakkyawan sakamako dole ne a tabbatar da su ta wata hanya dabam.

2) Bi da duk samfuran kamar masu iya kamuwa da cuta.Sanya safar hannu da tufafi masu kariya lokacin sarrafa samfurori.

3) Na'urorin da ake amfani da su don gwaji ya kamata a haɗa su da atomatik kafin a zubar.

4) Kar a yi amfani da kayan kit fiye da kwanakin ƙarewar su.

5) Kada a musanya reagents daga kuri'a daban-daban.

MISALI TATTAUNAWA DA AJIYA

1) Tattara samfuran Jini /Magunguna / Plasma gabaɗayan bin hanyoyin gwajin asibiti na yau da kullun.

2) Adana: Dukan Jini ba zai iya daskarewa ba.Ya kamata a sanya wani samfurin a cikin firiji idan ba a yi amfani da shi ba a ranar tarin.Ya kamata a daskare samfuran idan ba a yi amfani da su a cikin kwanaki 3 da tattara ba.Guji daskarewa da narke samfuran fiye da sau 2-3 kafin amfani.0.1% na Sodium Azide za a iya ƙarawa zuwa samfurin a matsayin mai kiyayewa ba tare da rinjayar sakamakon binciken ba.

HANYAR ASSAY

1) Yin amfani da ɗigon filastik da aka rufe don samfurin, ba da digo 1 (10μl) na Dukan Jini / Serum / Plasma zuwa samfurin madauwari na katin gwajin.

2) Ƙara digo 2 na Samfurin Diluent zuwa samfurin da kyau, nan da nan bayan an ƙara samfurin, daga diluent vial (ko duk abin da ke ciki daga ampul gwajin guda ɗaya).

3) Fassarar sakamakon gwaji a minti 15.