[YADDA AKE NUFI]

Gwajin gaggawa na mura A+B shine saurin immunoassay na gani don ƙwararru, ganowa da zato na mura A da B viral antigens suna samar da swabs na makogwaro da nasopharyngeal swab samfurori.An yi nufin gwajin don amfani da shi azaman taimako a cikin saurin ganewar cutar murar nau'in A da nau'in cutar B.

KA'IDA

Mura A+B Cassette na Gwajin gaggawa na gano mura A da B antigens ta hanyar fassarar gani na ci gaban launi akan tsiri.Kwayoyin rigakafin mura A da B ba su motsi a yankin gwajin A da B na membrane bi da bi.Yayin gwaji, samfurin da aka fitar yana amsawa da ƙwayoyin rigakafin mura A da B waɗanda aka haɗa su zuwa barbashi masu launi kuma an riga an riga an riga an rufe su a jikin samfurin gwajin.Cakuda sannan yayi ƙaura ta cikin membrane ta hanyar aikin capillary kuma yana hulɗa tare da reagents akan membrane.Idan akwai isassun antigens na mura A da B a cikin samfurin, band(s) masu launi za su fito a daidai yankin gwaji na membrane.Kasancewar rukunin launi a cikin yankin A da / ko B yana nuna sakamako mai kyau ga takamaiman antigens na hoto, yayin da rashi yana nuna sakamako mara kyau.Bayyanar band mai launi a yankin sarrafawa yana aiki a matsayin tsarin kulawa, yana nuna cewa an ƙara ƙarar samfurin da ya dace kuma an sami wicking membrane.

AJIYA DA KWANTA

1.A kit ya kamata a adana a 2-30 ° C har sai da ranar karewa buga a kan shãfe haske jakar.

2. Dole ne gwajin ya kasance a cikin jakar da aka rufe har sai an yi amfani da shi.

3. Kada a daskare.

4.Ya kamata a dauki kulawa don kare abubuwan da ke cikin kit daga gurɓatawa.Kada a yi amfani da shi idan akwai shaidar gurɓataccen ƙwayar cuta ko hazo.Cututtukan halittu na rarraba kayan aiki, kwantena ko reagents na iya haifar da sakamako na ƙarya.

TSARI

Kawo gwaje-gwaje, samfurori, da/ko sarrafawa zuwa zafin jiki (15-30°C) kafin amfani.

1. Cire gwajin daga jakar da aka rufe, kuma sanya shi a kan tsabta mai tsabta.Yi lakabin kaset tare da mai haƙuri ko ganewar sarrafawa.Don sakamako mafi kyau, yakamata a yi gwajin a cikin sa'a ɗaya.

2.Gently Mix Extraction reagent bayani.Ƙara digo 6 na Maganin Ciro a cikin bututun hakar.

3. Sanya samfurin swab na majiyyaci a cikin Tube Extraction.Mirgine swab aƙalla sau 10 yayin da ake danna swab a ƙasa da gefen Tube Extraction.Mirgine kan swab ɗin zuwa cikin bututun Cire yayin da kuke cire shi.Yi ƙoƙarin sakin ruwa mai yawa gwargwadon yiwuwa.Zubar da swab ɗin da aka yi amfani da shi daidai da ka'idar zubar da sharar biohazard.

4. Saka a kan tube tip, sa'an nan kuma ƙara 4 saukad da na fitar da samfurin a cikin samfurin da kyau.Kar a rike ko matsar da kaset ɗin gwajin har sai an gama gwajin kuma a shirye don karantawa.

5.Kamar yadda gwajin ya fara aiki, launi zai yi ƙaura a fadin membrane.Jira band(s) masu launin su bayyana.Ya kamata a karanta sakamakon a minti 10.Kar a fassara sakamakon bayan mintuna 20.

FASSARAR SAKAMAKO

Bada kaset ɗin gwaji da samfurori don daidaitawa zuwa zafin jiki (15-30 ℃ ko 59-86 ℉) kafin gwaji

1. Cire kaset ɗin gwaji daga jakar da aka rufe.

2. Juya bututun hakar samfurin, Rike da hakar samfurin

bututu a tsaye, canja wurin digo 3 (kimanin 100μl) zuwa samfurin.

da kyau (S) na kaset ɗin gwaji, sannan fara mai ƙidayar lokaci.Dubi hoton da ke ƙasa.

Jira layin masu launi su bayyana.Fassara sakamakon gwajin a cikin mintuna 15.Kar a karanta sakamakon bayan mintuna 20.

IYAKA NA GWAJI

1.The Flu A+B Cassette Mai Saurin Gwaji don ƙwararrun ƙwararrun ƙwararrun amfani da bincike ne, kuma yakamata a yi amfani da su kawai don gano ingancin mura A da/ko B.

2.Ba za a kafa ilimin etiology na kamuwa da cututtukan numfashi da ƙwayoyin cuta ke haifarwa banda mura A ko B virus da wannan gwajin.Kaset gwajin gaggawa na mura A+B yana da ikon gano ɓoyayyun ƙwayoyin mura da marasa ƙarfi.Ayyukan kaset gwajin gaggawa na mura A+B ya dogara da nauyin antigen kuma maiyuwa baya daidaita da al'adun tantanin halitta da aka yi akan samfurin iri ɗaya.

3.Idan sakamakon gwajin ya kasance mummunan kuma bayyanar cututtuka na asibiti ya ci gaba, ana ba da shawarar ƙarin gwaji ta amfani da wasu hanyoyin asibiti.Mummunan sakamako ba zai iya yin watsi da kasancewar mura A da/ko B a cikin samfurin, saboda suna iya kasancewa a ƙasa da ƙaramin matakin gano gwajin.Kamar yadda yake tare da duk gwaje-gwajen bincike, tabbatar da ganewar asali ya kamata likita ne kawai ya yi bayan an kimanta duk binciken asibiti da na dakin gwaje-gwaje.

4.Ba a tabbatar da ingancin kaset ɗin gwajin Flu A+B don ganowa ko tabbatar da ware al'adun tantanin halitta ba.

5. Rashin isassun samfurori ko rashin dacewa, tarawa, ajiya, da sufuri na iya haifar da sakamakon gwaji mara kyau.

6.Ko da yake an nuna wannan gwajin don gano ƙwayoyin cuta na mura na Avian, gami da cutar mura ta Avian A subtype H5N1, ba a san halayen aikin wannan gwajin tare da samfurori daga mutanen da suka kamu da H5N1 ko wasu ƙwayoyin cuta na Avian ba.

7. Performance halaye na mura A aka kafa a lokacin da mura A / H3 da A / H1 su ne predominant mura A ƙwayoyin cuta a wurare dabam dabam.Lokacin da wasu ƙwayoyin cuta na mura A ke fitowa, halayen aikin na iya bambanta.

8.Yara sukan zubar da kwayar cutar na tsawon lokaci fiye da manya, wanda hakan na iya haifar da bambance-bambancen hankali tsakanin manya da yara.

9.Kyawawan dabi'un tsinkaya masu kyau da mara kyau sun dogara sosai akan yaduwa.Sakamakon gwajin inganci na ƙarya yana da yuwuwa yayin lokutan ƙarancin aikin mura lokacin da yaɗuwar ya kasance matsakaici zuwa ƙasa.

NOTE:

1. Ƙarfin launi a cikin yankin gwaji (A / B) na iya bambanta dangane da ƙaddamar da nazarin da ke cikin samfurin.Saboda haka, duk wani inuwa na launi a cikin yankin gwaji (A/B) ya kamata a yi la'akari da shi mai kyau.Da fatan za a lura cewa wannan gwajin inganci ne kawai, kuma ba zai iya tantance yawan masu nazari a cikin samfurin ba.

2.Rashin isassun samfurin ƙira, tsarin aiki da ba daidai ba ko gwaje-gwajen da suka ƙare shine mafi kusantar dalilai na gazawar bandeji.