Na'urar Gwajin Dengue Ns1 (Dukkan JiniSerumPlasma)
Na'urar Gwajin Dengue Ns1 (Dukkan JiniSerumPlasma)
[YADDA AKE NUFI]
Dengue NS1 Antigen Rapid Test Cassette/Strip shine a gefe yana kwarara chromatographic immunoassay don gano ingancin antigens zuwa ƙwayoyin cuta na Dengue a cikin Dukan Jini/Serum/Plasma.Yana ba da taimako wajen gano kamuwa da cuta tare da ƙwayoyin cuta na Dengue.
[TAKATAI]
Zazzabin Dengue cuta ce mai saurin kamuwa da cuta mai saurin kamuwa da kwayar cutar dengue da sauro ke yadawa.Kwayar cutar Dengue na iya haifar da kamuwa da cuta, zazzabin dengue, zazzabin jini na dengue, zazzabin jini na dengue.Yawan bayyanar cututtuka na zazzabin dengue sun haɗa da farawa kwatsam, zazzabi mai zafi, ciwon kai, tsoka mai tsanani, kashi da ciwon haɗin gwiwa, kurjin fata, yanayin zubar jini, haɓakar kumburin lymph, rage yawan adadin jinin jini, thrombocytopenia da sauransu a wasu marasa lafiya.Wannan cuta m yana a wurare masu zafi da kuma subtropical yankin shahararsa, saboda wannan cuta ana daukar kwayar cutar ta hanyar sauro Aides, dalilin shahararsa yana da wasu seasonally, zama a kowace shekara yawanci a watan Mayu ~ Nuwamba, kololuwa ne a Yuli ~ Satumba.A cikin sabon yankin da ake fama da cutar, yawancin jama'a na iya kamuwa da cutar, amma abin da ya faru ya fi girma, a yankin da cutar ta fi kamari, yawanci yara ne.
[KA'IDA]
Dengue NS1 Antigen Rapid Test Cassette/Strip shine immunoassay bisa ka'idar fasahar rigakafin mutum-sandwich sau biyu.Yayin gwaji, anti-Dengue antibody ba ya motsi a cikin layin gwajin na'urar.Bayan an sanya cikakken jini/Magunguna/Plasma samfurin a cikin samfurin da kyau, yana amsawa da barbashi na anti-Dengue antibody wanda aka shafa akan kushin samfurin.Wannan cakuda yana yin ƙaura ta hanyar chromatographically tare da tsawon ɗigon gwajin kuma yana hulɗa tare da anti-Dengue antibody mara motsi.Idan samfurin ya ƙunshi antigen na dengue, wani layi mai launi zai bayyana a yankin layin gwajin yana nuna sakamako mai kyau.Idan samfurin bai ƙunshi antigen na kwayar cutar dengue ba, layin launi ba zai bayyana a wannan yanki yana nuna mummunan sakamako ba.Don yin aiki azaman sarrafa tsari, layi mai launi koyaushe zai bayyana a yankin layin sarrafawa wanda ke nuna cewa an ƙara ƙarar samfurin daidai kuma an sami wicking membrane.
[AJIYA DA KWANTAWA]
Ajiye kamar yadda aka kunshe a cikin jakar da aka rufe a zazzabi (4-30 ℃ ko 40-86 ℉).Kayan ya tsaya tsayin daka a cikin ranar karewa da aka buga akan lakabin.
Da zarar an buɗe jakar, yakamata a yi amfani da gwajin a cikin awa ɗaya.Tsawaita bayyanawa ga yanayin zafi da ɗanɗano zai haifar da lalacewar samfur.
An buga LOT da ranar karewa akan lakabin.
[SPECIMEN]
Za a iya amfani da gwajin don gwada Jini duka/Magunguna/Plasma.
Tattara samfurin jini (wanda ya ƙunshi EDTA, citrate ko heparin) ta hanyar huda jijiya ta hanyar daidaitattun hanyoyin gwaje-gwaje.
Rarrabe ruwan magani ko plasma daga jini da wuri-wuri don guje wa hemolytic.Yi amfani kawai bayyanannun samfuran da ba a rushe ba.
Ajiye samfurori a 2-8 ℃ (36-46 ℉) idan ba a gwada su nan da nan ba.Ajiye samfurori a 2-8 ℃ har zuwa kwanaki 7.Ya kamata a daskare samfuran a -20 ℃ (-4℉) don dogon ajiya.Kada a daskare duka samfuran jini.
Kauce wa daskare-daskare da yawa.Kafin gwaji, kawo daskararrun samfurori zuwa zafin daki a hankali kuma a gauraya a hankali.Ya kamata a fayyace samfuran da ke ƙunshe da ɓangarorin da ke bayyane ta hanyar centrifugation kafin gwaji.
Kada a yi amfani da samfuran da ke nuna babban ɗan layi, babban hemolytic ko turbidity don kauce wa tsangwama kan fassarar sakamako.
[TSARAR GWAJI]
- Bada na'urar gwaji da samfurori don daidaitawa zuwa zafin jiki (15-30 ℃ ko 59-86 ℉) kafin gwaji.
- [Don Tafi]
1. Cire tsiri na gwaji daga jakar da aka rufe kuma amfani da shi da wuri-wuri.
2. Sanya tsirin gwajin akan wuri mai tsabta da daidaitacce.
3. Don samfurin jini ko plasma: Riƙe digo a tsaye kuma canja wurin digo 3 na jini ko plasma (kimanin 100μl) zuwa gaɓoɓin samfurin tsiri na gwaji, sannan fara mai ƙidayar lokaci.Dubi hoton da ke ƙasa.
4. don samfuran jini duka: Riƙe digo a tsaye kuma canja wurin digo 1 na duka jini (kimanin 35μl) zuwa gaɓar samfurin gwajin, sannan ƙara digo 2 na buffer (kimanin 70μl) sannan a fara mai ƙidayar lokaci.Dubi hoton da ke ƙasa.
5. Jira layin (s) masu launi ya bayyana.Karanta sakamako a minti 15.Kar a fassara sakamakon bayan mintuna 20.
1.Cire kaset ɗin gwaji daga jakar da aka rufe kuma a yi amfani da shi da wuri-wuri.
2. Sanya kaset ɗin gwajin a kan tsaftataccen wuri mai tsabta.
3.Don samfurin jini ko plasma: Riƙe digo a tsaye kuma canja wurin digo 3 na jini ko plasma (kimanin 100μl) zuwa samfurin da kyau (S) na kaset ɗin gwaji, sannan fara mai ƙidayar lokaci.Dubi hoton da ke ƙasa.
4.Don cikakken samfuran jini: Rike digo a tsaye sannan a canja wurin digo 1 na duka jini (kimanin 35μl) zuwa samfurin rijiyar (S) na kaset ɗin gwaji, sannan ƙara digo 2 na buffer (kimanin 70μl) sannan a fara mai ƙidayar lokaci.Dubi hoton da ke ƙasa.
5. Jira layin (s) masu launi ya bayyana.Karanta sakamako a minti 15.Kar a fassara sakamakon bayan mintuna 20.
[FASARAR SAKAMAKO]
Tabbatacce:*Layi biyu sun bayyana.Layi mai launi ɗaya ya kamata ya kasance a cikin yankin sarrafawa (C), kuma wani layin launi na bayyane kusa yakamata ya kasance a cikin yankin gwaji (T).Wannan sakamako mai kyau yana nuna kasancewar antigens zuwa Dengue.
Korau: Layi mai launi ɗaya yana bayyana a yankin sarrafawa (C).Babu layi da ya bayyana a yankin gwaji (T).Wannan mummunan sakamako yana nuna rashin antigens zuwa Dengue.
Ba daidai ba: Layin sarrafawa ya kasa bayyana.Rashin isassun samfurin ƙira ko dabarun ƙa'ida ba daidai ba shine mafi kusantar dalilai na gazawar layin sarrafawa.Bita tsarin kuma maimaita gwajin ta amfani da sabon kaset/strip gwaji.Idan matsalar ta ci gaba, daina amfani da kuri'a nan da nan kuma tuntuɓi mai rarrabawa na gida.
