[YADDA AKE NUFI]

Dengue IgG/IgM Cassette Rapid Test Cassette wani nau'i ne na chromatographic immunoassay na gefe don gano ƙwararrun ƙwayoyin rigakafi (IgG da IgM) zuwa kwayar cutar dengue a cikin Dukan Jini / Serum / Plasma.Yana ba da taimako wajen gano kamuwa da cuta tare da ƙwayoyin cuta na Dengue.

[TAKATAI]

Zazzabin Dengue cuta ce mai saurin kamuwa da cuta mai saurin kamuwa da kwayar cutar dengue da sauro ke yadawa.Kwayar cutar Dengue na iya haifar da kamuwa da cuta, zazzabin dengue, zazzabin jini na dengue, zazzabin jini na dengue.Yawan bayyanar cututtuka na zazzabin dengue sun haɗa da farawa kwatsam, zazzabi mai zafi, ciwon kai, tsoka mai tsanani, kashi da ciwon haɗin gwiwa, kurjin fata, yanayin zubar jini, haɓakar kumburin lymph, rage yawan adadin jinin jini, thrombocytopenia da sauransu a wasu marasa lafiya.Wannan cuta m yana a wurare masu zafi da kuma subtropical yankin shahararsa, saboda wannan cuta ana daukar kwayar cutar ta hanyar sauro Aides, dalilin shahararsa yana da wasu seasonally, zama a kowace shekara yawanci a watan Mayu ~ Nuwamba, kololuwa ne a Yuli ~ Satumba.A cikin sabon yankin da ake fama da cutar, yawancin jama'a na iya kamuwa da cutar, amma abin da ya faru ya fi girma, a yankin da cutar ta fi kamari, yawanci yara ne.

[KA'IDA]

Dengue IgG/IgM Cassette Rapid Test Cassette ƙwaƙƙwarar ƙwayar cuta ce ta hanyar rigakafi don gano ƙwayoyin ƙwayoyin cuta na dengue (IgG da IgM) a cikin Dukan Jini / Serum / Plasma.Kaset ɗin gwajin ya ƙunshi: 1) wani kushin conjugate mai launin burgundy mai ɗauke da dengue recombinant envelope antigens conjugated da Colloid zinariya (dengue conjugates), 2) wani nitrocellulose membrane tsiri dauke da biyu gwajin Lines (IgG da IgM Lines) da kuma sarrafawa line (C line). ).An riga an riga an lulluɓe layin IgM tare da Mouse anti-Human IgM antibody, layin IgG an lulluɓe shi da Mouse anti-Human IgG antibody.Lokacin da aka ba da isasshen adadin samfurin gwaji a cikin rijiyar samfurin kaset ɗin, samfurin yana ƙaura ta hanyar aikin capillary a cikin kaset ɗin.IgM anti-dengue idan akwai a cikin samfurin zai ɗaure ga dengue conjugates.An kama immunocomplex ta reagent wanda aka lullube akan layin IgM, yana samar da layin IgM mai launin burgundy, yana nuna sakamakon gwajin IgM mai kyau kuma yana ba da shawarar sabon kamuwa da cuta.IgG anti-dengue, idan akwai a cikin samfurin, zai ɗaure zuwa dengue conjugates.Immunocomplex an kama shi ta hanyar reagent wanda aka riga aka rufawa akan rukunin IgG, yana samar da layin IgG mai launin burgundy, yana nuna sakamakon gwajin IgG mai inganci kuma yana ba da shawarar kamuwa da cuta kwanan nan ko maimaitawa.Rashin kowane layin T (IgG da IgM) yana nuna sakamako mara kyau.Don yin aiki azaman sarrafa tsari, layi mai launi koyaushe zai bayyana a yankin layin sarrafawa wanda ke nuna cewa an ƙara ƙarar samfurin daidai kuma an sami wicking membrane.

[AJIYA DA KWANTAWA]

Ajiye kamar yadda aka kunshe a cikin jakar da aka rufe a zazzabi (4-30 ℃ ko 40-86 ℉).Kayan ya tsaya tsayin daka a cikin ranar karewa da aka buga akan lakabin.

Da zarar an buɗe jakar, yakamata a yi amfani da gwajin a cikin awa ɗaya.Tsawaita bayyanawa ga yanayin zafi da ɗanɗano zai haifar da lalacewar samfur.

An buga LOT da ranar karewa akan lakabin.

[SPECIMEN]

Za a iya amfani da gwajin don gwada Jini duka/Magunguna/Plasma.

Don tattara cikakken jini, jini ko samfuran plasma bin hanyoyin gwajin asibiti na yau da kullun.

Rarrabe ruwan magani ko plasma daga jini da wuri-wuri don guje wa hemolytic.Yi amfani kawai bayyanannun samfuran da ba a rushe ba.

Ajiye samfurori a 2-8 ℃ (36-46 ℉) idan ba a gwada su nan da nan ba.Ajiye samfurori a 2-8 ℃ har zuwa kwanaki 7.Ya kamata a daskare samfuran a

-20 ℃ (-4℉) don dogon ajiya.Kada a daskare duka samfuran jini.

Kauce wa daskare-daskare da yawa.Kafin gwaji, kawo daskararrun samfurori zuwa zafin daki a hankali kuma a gauraya a hankali.Ya kamata a fayyace samfuran da ke ƙunshe da ɓangarorin da ke bayyane ta hanyar centrifugation kafin gwaji.

Kada a yi amfani da samfuran da ke nuna babban ɗan layi, babban hemolytic ko turbidity don kauce wa tsangwama kan fassarar sakamako.

[TSARAR GWAJI]

Bada na'urar gwaji da samfurori don daidaitawa zuwa zafin jiki (15-30 ℃ ko 59-86 ℉) kafin gwaji.

1. Kawo jakar zuwa dakin zafin jiki kafin bude shi.Cire kaset ɗin gwajin daga jakar da aka rufe kuma yi amfani da shi da wuri-wuri.

2. Sanya kaset ɗin gwajin a kan tsaftataccen wuri mai ma'auni.

3. Rike digo a tsaye kuma canja wurin digo 1 na samfurin (kimanin 10μl) zuwa samfurin da kyau (S) na kaset ɗin gwajin, sannan ƙara digo 2 na buffer (kimanin 70μl) kuma fara mai ƙidayar lokaci.Dubi hoton da ke ƙasa.

4. Jira layin (s) masu launi ya bayyana.Karanta sakamako a minti 15.Kar a fassara sakamakon bayan mintuna 20.