COVID-19 Neutralizing Antibody Rapid Test Cassette (Colloidal zinariya)
COVID-19 Neutralizing Antibody Rapid Test Cassette (Colloidal Gold) shine saurin immunoassay na chromatographic don gano ingantattun ƙwayoyin rigakafin ƙwayoyin cuta zuwa COVID-19 a cikin jinin ɗan adam, jini, ko plasma gabaɗaya a matsayin taimako a cikin tantancewar kasancewar antibodies. zuwa COVID-19.
Novel coronaviruses na cikin nau'in β.COVID-19 cuta ce mai saurin kamuwa da numfashi.Mutane gabaɗaya suna da sauƙi.A halin yanzu, marasa lafiya da suka kamu da cutar sankara ta coronavirus sune babban tushen kamuwa da cuta;Mutanen da suka kamu da asymptomatic suma suna iya zama tushen kamuwa da cuta.Dangane da binciken cututtukan cututtuka na yanzu, lokacin shiryawa shine kwanaki 1 zuwa 14, galibi daga kwanaki 3 zuwa 7.Babban bayyanar cututtuka sun haɗa da zazzabi, gajiya da bushewar tari.Ana samun cunkoso na hanci, hanci, ciwon makogwaro, myalgia da gudawa a wasu lokuta.
COVID-19 Neutralizing Antibody Rapid Test Cassette (Colloidal Gold) gwaji ne mai sauri wanda ke amfani da hadewar S-RBD antigen ruɓaɓɓen barbashi don gano kawar da ƙwayoyin rigakafi zuwa COVID-19 a cikin jinin ɗan adam, jini, ko plasma baki ɗaya.
The COVID-19 Neutralizing Antibody Rapid Test Cassette (Colloidal Gold) wani ingantaccen membrane ne wanda ke tushen immunoassay don gano kawar da ƙwayoyin rigakafi zuwa COVID-19 a cikin jini gaba ɗaya, jini ko plasma.An riga an riga an riga an yi rufin membrane tare da Angiotensin I Converting Enzyme 2 (ACE2) akan layin gwaji na tsiri.Yayin gwaji, duka jini, jini ko samfurin plasma suna amsawa da S-RBD conjugated colloid zinariya.Cakuda yana ƙaura zuwa sama akan membrane chromatographically ta aikin capillary don amsawa tare da ACE2 akan membrane kuma ya haifar da layi mai launi.Kasancewar wannan layi mai launi yana nuna sakamako mara kyau, yayin da rashinsa yana nuna sakamako mai kyau.Don zama mai sarrafa tsari, layi mai launi koyaushe zai canza daga Blue zuwa Ja a cikin yankin layin sarrafawa, yana nuna cewa an ƙara ƙarar samfurin da ya dace kuma an sami wicking membrane.
| Na'urorin gwaji da aka cika daban-daban | Kowace na'ura tana ƙunshe da tsiri mai launin conjugates da reactive reagents waɗanda aka riga aka watsa a yankuna masu dacewa. |
| pipettes masu zubarwa | Don ƙara samfurori amfani |
| Buffer | Phosphate buffered saline da preservative |
| Saka kunshin | Don umarnin aiki |
An Samar da Kayayyakin
| ●Na'urorin gwaji | ●Masu jefarwa |
| ●Buffer | ●Abin da aka saka |
Kayayyakin da ake buƙata Amma Ba a Samar da su ba
| ●Kwayoyin tattara samfurori | ●Lokaci |
| ●Centrifuge |
1. Don ƙwararrun in vitro diagnostic amfani kawai.
2. Kada ku yi amfani da bayan ranar karewa da aka nuna akan kunshin.Kada kayi amfani da gwajin idan jakar jakar ta lalace.Kada a sake amfani da gwaje-gwaje.
3. Maganin hakar reagent yana ƙunshe da maganin gishiri idan maganin ya tuntuɓi fata ko ido, ja da ruwa mai yawa.
4. Guje wa ƙetare gurɓata samfuran ta amfani da sabon kwandon tattara samfuran ga kowane samfurin da aka samu.
5. Karanta dukan hanya a hankali kafin gwaji.
6. Kada ku ci, ku sha ko shan taba a wurin da ake sarrafa samfurori da kayan aiki.Yi amfani da duk samfuran kamar suna ɗauke da ƙwayoyin cuta.Kula da ƙayyadaddun ƙayyadaddun ƙayyadaddun ƙayyadaddun ƙayyadaddun ƙayyadaddun ƙwayoyin cuta a duk tsawon aikin kuma bi ƙa'idodin ƙa'idodi don zubar da samfuran da suka dace.Sanya tufafi masu kariya kamar sutturar dakin gwaje-gwaje, safar hannu da za'a iya zubarwa da kariyar ido lokacin da aka tantance samfurori.
7. Idan ana zargin kamuwa da cuta tare da novel coronaviruses bisa la'akari da halin yanzu asibiti da kuma epidemiological ka'idojin dubawa shawarar da hukumomin kiwon lafiya na jama'a, ya kamata a tattara samfurori tare da dace kamuwa da cuta kariya kariya ga sabon coronaviruses da aika zuwa jiha ko na gida sashen kiwon lafiya don gwaji.Bai kamata a yi ƙoƙarin al'adar ƙwayar cuta ba a waɗannan lokuta sai dai idan akwai BSL 3+ don karɓa da samfuran al'adu.
8. Kada ka musanya ko Mix reagents daga daban-daban kuri'a.
9. Danshi da zafin jiki na iya cutar da sakamako mara kyau.
10. Ya kamata a jefar da kayan gwajin da aka yi amfani da su daidai da dokokin gida.
1. Ya kamata a adana kit ɗin a 2-30 ° C har sai an buga ranar ƙarewa a kan jakar da aka rufe.
2. Dole ne gwajin ya kasance a cikin jakar da aka rufe har sai an yi amfani da shi.
3. Kar a daskare.
4. Ya kamata a kula don kare abubuwan da ke cikin kayan daga gurɓatawa.Kada a yi amfani da shi idan akwai shaidar gurɓataccen ƙwayar cuta ko hazo.Cututtukan halittu na rarraba kayan aiki, kwantena ko reagents na iya haifar da sakamako na ƙarya.
Yi la'akari da kowane kayan asalin ɗan adam a matsayin masu kamuwa da cuta kuma sarrafa su ta amfani da daidaitattun hanyoyin kare lafiyar halittu.
Dukan Jini na Capillary
Wanke hannun mara lafiya sannan a bar shi ya bushe.Tausa hannu ba tare da taɓa huda ba.Huda fata tare da bakararre lancet.Goge alamar jini na farko.A hankali shafa hannun daga wuyan hannu zuwa tafin hannu zuwa yatsa don samar da digon jini mai zagaye akan wurin huda.Ƙara samfurin Jini na Yatsu zuwa na'urar gwaji ta amfani da bututun capillary ko rataye digo.
venous Duk Jinin:
Tattara samfurin jini a cikin bututu mai tarin shuɗi ko kore (mai ɗauke da EDTA, citrate ko heparin, bi da bi a cikin Vacutainer®) ta hanyar jijiya.
Plasma
Tattara samfurin jini a cikin bututu mai tarin shuɗi ko kore (mai ɗauke da EDTA, citrate ko heparin, bi da bi a cikin Vacutainer®) ta hanyar jijiya.Ware plasma ta hanyar centrifugation.Cire plasma a hankali cikin sabon bututu da aka riga aka yi wa lakabin.
Magani
Tattara samfurin jini a cikin babban bututun tarin ja (wanda ba ya ɗauke da maganin rigakafi a cikin Vacutainer®) ta hanyar jijiya.Izinin jinin ya toshe.Ware maganin ta hanyar centrifugation.Cire maganin a hankali cikin sabon bututu da aka riga aka yi wa lakabi da shi.
Gwada samfurori da wuri-wuri bayan tattarawa.Ajiye samfurori a 2°C-8°C idan ba a gwada su nan da nan ba.
Ajiye samfurori a 2 ° C-8 ° C har zuwa kwanaki 5.Ya kamata a daskare samfuran a -20 ° C don dogon ajiya.
Kauce wa daskare-daskare da yawa.Kafin gwaji, kawo daskararrun samfurori zuwa zafin daki a hankali kuma a gauraya a hankali.Ya kamata a fayyace samfuran da ke ɗauke da ɓangarorin ɓangarorin da ke bayyane ta hanyar centrifugation kafin gwaji.Kar a yi amfani da samfuran da ke nuna babban lipemia, babban hemolysis ko turbidity don guje wa tsangwama kan fassarar sakamako.
Kawo samfurin da kayan aikin gwadawa zuwa zafin ɗaki Haɗa samfurin da kyau kafin a tantance da zarar an narke.Sanya na'urar gwajin akan tsaftataccen wuri mai lebur.
Don samfurin jini duka:
Don amfani da bututun capillary: Cika bututun capillary dacanja wurin kusan 50µL (ko 2 digo) na yatsa gabaɗayan jinisamfurin ga samfurin rijiyar (S) na na'urar gwaji, sannan ƙara1 digo (kimanin 30 µl)naSamfurin Diluentnan da nan cikin samfurin rijiyar.
Ga cikakken samfurin jini:
Cika digo da samfurin sannancanja wurin 2 saukad (kimanin 50 µl)na samfurin a cikin rijiyar samfurin.Tabbatar da cewa babu kumfa na iska.Sannancanja wurin digo 1 (kimanin 30 µl)na Samfurin Diluent nan da nan a cikin rijiyar samfurin.
Samfurin Plasma/Serum:
Cika digo da samfurin sannancanja wurin digo 1 (kimanin 25 µl)na samfurin a cikin rijiyar samfurin.Tabbatar da cewa babu kumfa na iska.Sannancanja wurin digo 1 (kimanin 30 µl) na Samfurin Diluent nan da nan a cikin rijiyar samfurin.
Saita mai ƙidayar lokaci.Karanta sakamakon a minti 15.Kar a karanta sakamakon bayan20 mintuna.Don guje wa rudani, jefar da na'urar gwajin bayan fassarar sakamakon
| SAKAMAKO MAI KYAU:
| Ƙungiya mai launi ɗaya kawai ya bayyana a cikin yankin sarrafawa (C).Babu makaɗa mai launi da ya bayyana a yankin gwaji (T). |
| SAKAMAKO MAI KYAU:
| Makada masu launi biyu suna bayyana akan membrane.Ƙungiya ɗaya yana bayyana a cikin yankin sarrafawa (C) kuma wani rukunin yana bayyana a yankin gwaji (T). |
| * NOTE: Ƙarfin launi a yankin layin gwajin zai bambanta dangane da yawan abubuwan da ke kawar da ƙwayoyin cuta zuwa COVID-19 a cikin samfurin.Sabili da haka, duk wani inuwa na launi a cikin yankin layin gwaji ya kamata a yi la'akari da shi mara kyau. | |
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Sakamako mara inganci:
| Ƙungiyar sarrafawa ta kasa bayyana.Sakamako daga kowane gwajin da bai samar da rukunin sarrafawa ba a ƙayyadadden lokacin karantawa dole ne a yi watsi da shi.Da fatan za a sake duba tsarin kuma a maimaita tare da sabon gwaji.Idan matsalar ta ci gaba, daina amfani da kit ɗin nan da nan kuma tuntuɓi mai rarrabawa na gida. |
1. Ikon Ciki:Wannan gwajin ya ƙunshi ginanniyar fasalin sarrafawa, ƙungiyar C.Layin C yana tasowa bayan ƙara samfuri da samfurin diluent.In ba haka ba, sake duba gabaɗayan hanya kuma maimaita gwaji tare da sabuwar na'ura.
2. Ikon Waje:Kyakkyawan dakin gwaje-gwaje na kyau yana bada shawarar amfani da sarrafawar waje, tabbatacce kuma mara kyau (wanda aka bayar akan buƙata), don tabbatar da dacewa yin assay.
